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Next generation
sequencing

Empower your lab with fast, reliable sequencing results. Whether you're investigating cancer, tracking genetic variation, or discovering new biomarkers, our customizable NGS portfolio and streamlined workflows deliver the answers you need.

Oligos & Reagents

Empower your lab with fast, reliable sequencing results. Whether you're investigating cancer, tracking genetic variation, or discovering new biomarkers, our customizable NGS portfolio and streamlined workflows deliver the answers you need.

Next generation
sequencing

Empower your lab with fast, reliable sequencing results. Whether you're investigating cancer, tracking genetic variation, or discovering new biomarkers, our customizable NGS portfolio and streamlined workflows deliver the answers you need.

Next generation
sequencing

Empower your lab with fast, reliable sequencing results. Whether you're investigating cancer, tracking genetic variation, or discovering new biomarkers, our customizable NGS portfolio and streamlined workflows deliver the answers you need.

Next generation
sequencing

Empower your lab with fast, reliable sequencing results. Whether you're investigating cancer, tracking genetic variation, or discovering new biomarkers, our customizable NGS portfolio and streamlined workflows deliver the answers you need.
Home Products & Services Catageory Template
Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. Reliable, comprehensive genomic information is provided via a streamlined workflow, powered by patented AMP chemistry and Archer Analysis’s user-friendly bioinformatics platform. Flexibility with assay content, reagent formats, and analysis settings ensures there is an NGS assay solution for your lab’s unique needs.

Looking for a custom solution?

Optimize your oligo quality with our in-house synthesis process

IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.
EXPLORE our products

Optimize your oligo quality with our in-house synthesis process

IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.
EXPLORE our products

Inspiring discoveries
that transform lives

"We have been very impressed with the characteristics of Alt-R™ HiFi Cas9 Nuclease, this version consistently gives us high on-target editing activity while having low off-target activity.
We are excited to use it in our future experiments developing novel genome editing based therapies for severe diseases."

Matt Porteus

MD, PhD Title, Company

Product
comparison table

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Key Specifications
Cas9 System
Cas12a System
Applications General Genome Editing
  • For species with AT-rich genomes
  • For regions with limiting design space for use of the CRISPR-Cas9 system
Ribonucleoprotein components
  • gRNA options:
    1. crRNA and tracrRNA
    2. sgRNA
  • Cas9 endonuclease
  • crRNA
  • Cas12a endonuclease
Variants
  • Wild type
  • HiFi
  • Nickases (H840A and D10A)
  • Wild type
  • Ultra (Improved performance)
Cas9 crRNA:tracrRNA (option 1)
crRNA
  • Native: 42 nt
  • Alt-R: 35-36 nt (36 nt recommended)
tracrRNA
  • Native: 89 nt
  • Alt-R: 67 nt
 __
Cas9 sgRNA (option 2)
  • Alt-R: 99-100 nt (100 nt recommended)
 __
Cas12a crRNA __
  • Native: 42-44 nt
  • Alt-R: 40-44 nt (41 nt recommended)
CRISPR enzyme
  • Class 2, Cas type II
  • M.W.*: 162,200 g/mol
  • Endonuclease domains: RuvC-like and HNH
  • Class 2, Cas type V
  • M.W.*: 156,400 g/mol
  • Endonuclease domain: RuvC-like only
DNA cleavage
  • Wild-type and HiFi: Blunt-ended cut 3 bases upstream of the protospacer sequence
  • D10A nickase with paired gRNAs: 5’ overhang
  • H840A nickase with paired gRNAs: 3’ overhang
  • PAM site often destroyed during genome editing
  • 5’ overhanging cut on the 5’ side of the protospacer sequence
  • PAM site may be preserved after genome editing
PAM sequence
  • NGG
  • TTTV for Cas 12a V3
  • TTTN for Cas12a Ultra
Current recommendations for Alt-R RNP delivery
  • Lipid-mediated transfection
  • Electroporation (Alt-R enhancer recommended)
  • Microinjection
  • Electroporation (Alt-R enhancer recommended)
  • Microinjection
Quick comparison of Cas9 vs. Cas12a genome editing complexes

Explore other IDT solutions

IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.
IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.

DNA Oligo full-length product coupling efficiency

IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.
IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.

DNA Oligo full-length product coupling efficiency

IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.
IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides. IDT proprietary platforms have better coupling efficiency than other suppliers, ensuring your order contains more full-length oligonucleotides.