Deliver answers confidently using Archer’s proprietary chemistry, streamlined workflow, and user-friendly analysis.
Archer technology was designed as an NGS assay solution—which means it has inherent flexibility across different sample types (i.e. FFPE, fresh frozen, cytology smear, fine needle aspirate, and blood), nucleic acid inputs (i.e., DNA, RNA, and ctDNA), panel content, and genomic alterations (i.e. SNVs, indels, CNVs, MSI, and TMB). It also means easier adoption, training, and continued research use in your lab with multiple Archer panels.
Accurate and more comprehensive identification of relevant genomic alterations informed the design of AMP chemistry primers to create high performance next generation sequencing assays.
AMP chemistry includes Molecular Barcode Adapters, which are ligated before PCR. These adapters include a molecular barcode (MBC) - the unique molecular identifier (UMI) sequence which enables molecular tracking and error correction. MBC adapters also include a sample index region for multiplex sequencing. Lastly, a universal primer binding site supports novel fusion detection and highly sensitive amplification of degraded samples relevant to blood cancer and solid tumor research applications. Nested PCR employed in AMP chemistry increases specific amplification of targeted regions.
AMP primers function independently, which means gene content can easily be customized without compromising assay performance. Archer panels maintain primer uniformity, coverage uniformity, and on-target percent regardless of primer pool size [1]*.
*Read depth must be scaled appropriately.
AMP chemistry was designed to amplify targets effectively even when quality and quantity of DNA, RNA, or ctDNA is low. The chemistry and analysis software reduce background noise and increases the sensitivity for detection of low-allelic fraction mutations, while retaining specificity [1]. This allows for wide sample type compatibility, including sources with low quality nucleic acid input such as those from degraded FFPE tissue important in solid tumor research applications.
Archer NGS library preparation is simple, fast, and designed to minimize potential user errors for your lab.
Library preparation workflows can be run in parallel, while sample indexing allows for all Archer libraries to be sequenced together. Flexible run sizes are available with choice of lyophilized reagents in breakaway 8-tube strips, allowing for runs as small as one sample, or 96-well breakaway plates for larger runs. Scalable, high-throughput, liquid reagents are available in single-use 24- and 96-reaction kits.
Single-use, reaction-sized lyophilized reagents allow your lab to avoid potential contamination during manual pipetting and provide extended shelf life. Alternatively, liquid reagents enable automated liquid handling workflows for high volume research labs. Availability of both lyophilized and liquid reagents allows you to choose the best fit for your lab. Regardless of format, Archer reagents are color-coded with matched protocol steps for easier benchwork.
Workflows are designed to balance optimal performance with fast turnaround time, with most requiring only 3.5 hours of hands-on time and 1.5 days from nucleic acid to sequencer loading.
Archer Analysis is a versatile bioinformatics platform that streamlines analysis for all Archer assays and is available as a hosted service, or as a locally installed server.
Get answers rather than raising more questions with integrated sample and variant QC metrics, error correction, comprehensive read statistics, and clear visualized outputs. Analysis can be integrated with LIMS data, third-party or locally-curated annotations, while providing customizable PDF outputs.
Keep your data safe with a private cloud-based instance, or a virtual machine that can be installed on your own hardware, behind your firewall. Advanced permissions-based access also ensures another layer of security. Additionally, IDT passed a third-party HIPAA audit, and Archer Analysis Unlimited is compliant with all associated standards.*
Handle hundreds of samples simultaneously with rapid analysis times without expensive hardware or associated maintenance when you use Archer’s Analysis Unlimited, a private cloud-based solution.
*The Health Insurance Portability and Accountability Act (HIPAA) establishes standards for the security and privacy of protected health information.