DNA Oligonucleotides

Ordering

• Complete confidence in oligos that are checked by electrospray ionization mass spectrometry (ESI-MS)*
• Begin your project sooner with >90% of orders shipped within 24 hours^†
• Extensive control of oligo specifications with custom formulation and mixing options

*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.
†Small scale, standard, desalted oligos

Product Details

DNA Oligonucleotides are short, single- and double-stranded synthetic DNA sequences that can be used in a variety of molecular biology applications. Using the Oligo Entry ordering tool, you can design your oligos to contain any DNA sequence you require and select from hundreds of modifications to suit your specific research needs.

Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-MS*, and quantified twice by UV spectrophotometry to provide yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI -MS.

For standard, desalted oligos ≥20 bases, we offer the following yield guarantees:

*Guaranteed yield for unmodified oligos 25–50 bases; will vary with oligo composition, length, and purification.

Product Data

IDT's long-standing reputation as a pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure fast turnaround times. Our ability to control these variables allows us to consistently produce high-quality oligos for use in routine and specialized applications.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes result in high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in a reliable product that helps provide confidence in your research.

Services

The following services represent our most common offerings. If your project requires a service not listed here, contact us and we will work with you individually to accommodate your request.

Purification

For demanding applications such as multiplex PCR, cloning, mutagenesis, or antisense/RNAi methods, additional purification may be needed for your oligonucleotides. All purified oligos up to 60 bases receive a purity guarantee via QC by capillary electrophoresis. Learn more »

Formulation

Specific research requirements and individual preferences require oligos that can be delivered in unique formats. With our formulation and packaging services, you can have your oligos manufactured to your specific requirements. Learn more »

Custom quality control

In addition to the free ESI-MS analysis provided for all oligos, your project may require one of the analytical services listed below. Learn more »

Large-scale synthesis

Custom DNA Oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are ISO 13485:2016 certified, or if you are interested in our third-party manufacturing services, please click here.

Frequently asked questions