Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location. We offer 3 gRNA formats: crRNA:tracrRNA duplex, crRNA XT:tracrRNA duplex, and single guide RNA (sgRNA).

To be used with tracrRNA to form a functional gRNA duplex. Suitable for most applications. Contains chemical modifications to protect from degradation by cellular RNases.

To be used with tracrRNA to form a functional gRNA duplex. Suitable for challenging experimental conditions (e.g., high nuclease environments or with Cas9 mRNA). Contains additional chemical modifications compared to crRNA to provide a cost-effective option for increased functional stability.

Single RNA molecules comprised of both crRNA and tracrRNA sequences. Suitable for challenging experimental conditions (e.g., high nuclease environments or with Cas9 mRNA). Contains chemical modifications for a high level of functional stability.

We guarantee* our predesigned guide RNAs targeting human or mouse, rat, zebrafish, or nematode genes. For other species, we recommend using our proprietary algorithms that enable the design of custom guide RNAs.

Note: If you have protospacer designs of your own, or designs from publications, use our design checker tool to assess their on- and off-targeting potential before ordering guide RNAs that are synthesized with using our Alt-R gRNA modifications.

If you have protospacer designs of your own, or designs from publications, we recommend using the design checker tool to assess on- and off-targeting potential before ordering guide RNAs that are synthesized with our Alt-R guide RNA gRNA modifications. For designs that do not require this analysis, you can directly order any user-defined crRNA or sgRNA with our tube and plate ordering buttons.

Universal 67mer tracrRNA that contains proprietary chemical modifications conferring increased nuclease resistance. Hybridizes to crRNA to activate the Cas9 enzyme. More info »