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John Tossberg

John Tossberg received a Bachelor of Science in biology from Bates College and a Master of Science from Iowa State University, where he studied equine infectious anemia virus. For the last 15 years, John has worked at Vanderbilt University Medical Center, first developing RNA-based diagnostic tests for autoimmune diseases and most recently studying how endogenous Alu RNAs interact with the immune system. Currently, he is investigating immunologically active Alu RNAs as a possible therapeutic.

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A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates

Since the start of molecular biology, plasmid-based cloning has been an essential component of both basic and medical research. A limitation is that plasmid-based cloning is a complicated, multistep process.

This publication presents a new method preparing synthetic genes and mature mRNA that can be efficiently introduced into cells and translated into functional proteins without the need for plasmid-based cloning, other RNA such as long noncoding RNA, can also be prepared using this method.

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