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How to design primers and probes for PCR and qPCR

How to design primers and probes for PCR and qPCR

Many factors can influence PCR experiments, including primer and probe characteristics such as location, length, interaction and self-folding, melting temperature, annealing temperature, and GC content. Review these general recommendations for designing primers and probes and for choosing target locations for PCR amplification.

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IDT’s commitment to biosecurity

IDT’s commitment to biosecurity

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Explaining multiple peaks in qPCR melt curve analysis

Explaining multiple peaks in qPCR melt curve analysis

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Tips for resuspending and diluting your oligonucleotides

Tips for resuspending and diluting your oligonucleotides

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