Calculators for resuspension and dilution of oligonucleotides

Figure 1. Access the Resuspension and Dilution Calculators via the Tools tab on the IDT website.

Resuspension Calculator

The Resuspension Calculator (Figure 2) determines the volume needed to resuspend a dry-shipped oligo for a desired final concentration. You can provide the input as nmol, mg, or as OD₂₆₀ with MW or extinction coefficient (provided on the spec sheet sent with the oligo). Likewise, you can specify the units of final concentration.

Note: Be sure to enter the amount shipped, not the scale ordered, as synthesis efficiency and purification will decrease the ultimate amount of oligo you receive.

Figure 2. The IDT Resuspension Calculator.

Dilution Calculator

Figure 3. The IDT Dilution Calculator.

The Dilution Calculator (Figure 3) provides details on how to dilute a stock solution to a desired concentration. You are given a choice of input and output concentration units (molarity or mass per volume). The Resuspension and Dilution Calculators are just two examples out of a suite of SciTools™ programs, provided for free use on the IDT website. Others include:

• OligoAnalyzer Tool—provides primer properties like T_m and GC%, and predicts primer loops, primer dimers, and primer-primer compatibility
• UNAFold Tool—predicts nucleic acid folding and hybridization
• Tools for selecting qPCR assays and DsiRNAs from a library of designs
• Design engines for qPCR primers and probes, RNAi, antisense, and locked nucleic acid sequences

Explore these tools, and others, in the SciTools suite here.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations. RUO23-1868_001