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Amplicon sequencing is a method that targets distinct regions of a genome for sequencing. The method uses highly multiplexed pairs of primers that flank the desired regions of the genome. The use of primer pairs for targeted regions provides the ability to adjust the length of each amplicon for the specific sequencing instrument. In addition, the primers can be adjusted to avoid areas of the genome with high GC base pairs or secondary structures. The method is best used for understanding genetic variation, which makes it amenable to agricultural, environmental, and medical research applications.
The xGen family of predesigned amplicon panels makes it easy for you to identify genetic variation such as single nucleotide variations (SNVs), single nucleotide polymorphisms (SNPs), insertions/deletions (indels), etc. For research in:
IDT also offers the xGen Human Sample ID Amplicon Panel
In each of the xGen Amplicon Panels, the PCR primers are manufactured with the IDT proprietary DNA synthesis technology. The panels have been tested to ensure the primers amplify the targets with few dropouts.
Amplicon sequencing panels can also be expanded by adding xGen Custom Amplicon panels with primers that target your genes of interest.
In addition to the panel of multiplexed PCR primers, the following reagents are required for the amplicon sequencing workflow:
For the exact reagents needed, refer to the individual xGen Predesigned Panels ordering pages.