Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
For best results, use the cycling parameters provided in the PrimeTime One-Step 4X Broad-Range Master Mix Protocol.
Fast cycling conditions: 15 min. 50°C; 30 sec. 95°C; 35–45x (5 sec. 95°C, 45 sec. 60°C).