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What is Normalase™?
Normalase enzymatically generates equimolar library pools and balanced sample representation in sequencing. First, the Normalase I reaction is performed on each sample individually. Next, libraries are pooled with equal volumes and the Normalase II reaction normalizes all samples within the pool to 4 nM, resulting in an equimolar library pool.
The protocol is designed for SNAP libraries that produce consistent amplified library yields of ≥6 nM or ≥12 nM following indexing PCR. Most samples processed with the SNAP protocol produce amplified library yields of 12 nM or greater; however, if you are concerned that all samples will not reach 12 nM, the Normalase reaction can be adjusted such that a minimum of 6 nM per sample is required and the library pool will be normalized down to 2 nM.