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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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There are no PAM NGG sequences close to my locus of interest. What can I do?
If the region of interest is so narrow that there are no “GG” motifs, you may have to explore Cas9 endonucleases (from other organisms) that recognize different PAM sites. [Jiang et al. (2013) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotech, 31:233–239.] has also shown that S. pyogenes Cas9 recognizes NAG, though with less efficiency. Note that our research group is not focused on testing these approaches.