Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Search all FAQs:
Is the PAM sequence part of the gRNA sequence construct?
.png?sfvrsn=58c63b07_0 "pam-site-callout (1)")
Cas9 nuclease, derived from S. pyogenes, recognizes a PAM sequence of NGG that is found directly downstream of the target sequence in the genomic DNA, on the non-target strand.
Want to Learn More?
Download The CRISPR Basics Handbook that covers applications of CRISPR technology from guide RNA design to data analysis.
The latest CRISPR abbreviations and definitions in a single list!
Design custom solutions for CRISPR genome editing.
References:
1. Anders C, Niewoehner O, Duerst A, Jinek M. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature. 2014;513(7519):569-573.