Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Calculate the appropriate resuspension volume for your oPools product with the IDT Resuspension Calculator.
First, convert the oPool scale—provided in pmol—to a nmol quantity.
If your desired final concentration is µM per oligo, use this equation to determine your quantity:
pmol per oligo / 1000 = # nmol per oligo
Example 1. Final concentration of 1 µM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 1):
Figure 1. 50 pmol oPools Oligo Pool resuspended to 1 µM per oligo.
If your desired final concentration is nM per oligo, you can convert using this equation:
nM concentration / 1000 = # µM
Example 2. Final concentration of 100 nM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration in µM: 100 nM / 1000 = 0.1 µM
Resuspension calculator (Figure 2):
Figure 2. 50 pmol oPools Oligo Pool resuspended to 100 nM per oligo.
If your desired final concentration is per pool, use this equation:
(pmol per oligo x # of oligos in pool) / 1000 = # nmol per pool
Example 3. Final concentration of 1 µM per pool.
Scenario: 50 pmol oPools Oligo Pool containing 100 oligos
Quantity: (50 pmol x 100 oligos) / 1000 = 5 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 3):
Figure 3. 50 pmol oPools Oligo Pool containing 100 oligos resuspended to 1 µM per pool.