The rhAmpSeq™ CRISPR Analysis System—an end-to-end solution for genome editing quantification

CRISPR systems enable targeted editing in a wide variety of organisms by introducing single- or double-strand breaks (DSBs) to DNA, which can be leveraged to introduce genomic changes. However, unintended DSBs can occur off-target at genomic loci with partial complementarity to CRISPR-Cas guide RNA (gRNA) sequences, resulting in genotype/phenotype changes in those edited cells.  

Genome editing at on- and off-target sites can be quantified with multiplexed targeted next-generation sequencing (NGS) but requires bioinformatics expertise to analyze. Here, we present the rhAmpSeq CRISPR Analysis System as a streamlined end-to-end solution for designing assays, generating sequencing libraries, and analyzing NGS data for quantifying CRISPR on- and off-target editing effects without the need for bioinformatics expertise. 

Objectives

• Learn how the rhAmpSeq CRISPR Analysis System can help you streamline your CRISPR genome editing analysis
• Learn how to simultaneously quantify intended edits and evaluate off-target modifications with accuracy
• Learn about reducing CRISPR off-target effects without compromising potency

RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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